• The generation of hESCs involves numerous reagents including

  2018-10-22

  

  The generation of hESCs involves numerous reagents, including growth factors, small molecules, and media. To avoid infection by animal-sourced components, mouse feeder jnk inhibitors can be substituted by human fibroblasts (Ellerstrom et al., 2006; Genbacev et al., 2005). Chemically defined hESC cu

• br Results br Discussion Culturing hFK in mNPEM instantly

  2018-10-22

  

  Results Discussion Culturing hFK in mNPEM instantly showed morphological heterogeneity, including specific undifferentiated stem cell niches and differentiating epithelial cells, which could be further exploited to show an EpCAM expression gradient (EpCAM−, EpCAMdim, EpCAMbright) within the N

• As PcG proteins and miRNAs are usually co expressed

  2018-10-22

  

  As PcG proteins and miRNAs are usually co-expressed in many cell types or cancer tissues, such as keratinocytes (Eckert et al., 2011; Yi et al., 2008), hematopoietic stem interleukin 1 receptor antagonist (Takamatsu-Ichihara and Kitabayashi, 2016), hepatocellular carcinoma (Yonemitsu et al., 2009),

• We also showed that the

  2018-10-22

  

  We also showed that the transient suppression of Nanog by siRNA annulled the self-renewal capacity and tumorigenic activity of GSCs and induced the differentiation of these cells. Recent evidence suggested a shared genomic fingerprint between ESCs, cancer cells, and cancer stem cells. Nanog, Sox2, a

• br Conflict of interest statement The following are the supp

  2018-10-22

  

  Conflict of interest statement The following are the supplementary data related to this article. Acknowledgments This research was supported by funds to R.C. and F.C. from the Italian Ministry of Education, University and Research (MIUR). Introduction Besides hematopoietic stem hiv protea

• Although p NTR has been

  2018-10-22

  

  Although p75NTR has been correlated to the proliferation and differentiation of NSCs, the underlying molecular mechanisms remain undefined. ERK 1/2 activation has been shown to be responsible for the proliferation and oligodendrogenesis of NSCs, and to be able to promote LY2874455 progression and Ol

• Chondrogenic differentiation in our model is affected by der

  2018-10-22

  

  Chondrogenic differentiation in our model is affected by deregulation of LMNA, especially in PG-MSCs, as revealed by a lowered COL2/COL1 ratio and lower expression of COL2 and AGG as shown by real-time RT-PCR. While our findings do not completely agree with the results of the study by Scaffidi and M

• br Materials and methods br Results br Discussion Following

  2018-10-22

  

  Materials and methods Results Discussion Following the demonstration that a ROCK inhibitor such as Y27632 permits the survival of single-cell hESCs (Watanabe et al., 2007), several groups utilized it to establish suspension culture systems for the expansion of hPSCs based on single-cell ino

• The intestinal epithelium is a particularly attractive biolo

  2018-10-20

  

  The intestinal epithelium is a particularly attractive biological adult model to study epigenetic mechanisms driving differentiation in vivo. In the intestinal epithelium, two populations of SC have already been described and isolated (Lgr5+ and Bmi1+ cells; Barker et al., 2007; Sangiorgi & Capecchi

• Several groups have reported the culture

  2018-10-20

  

  Several groups have reported the culture and differentiation of fetal or adult neural stem Epinephrine Bitartrate (NSCs) on polymer-based substrates (Park et al., 2014; Little et al., 2008; Bhang et al., 2007; Lundin et al., 2011; Saha et al., 2007). It is important to point out that the NSCs used

• In accordance with our previous observations Salazar

  2018-10-20

  

  In accordance with our previous observations (Salazar et al., 2010), hCSN-SCns transplanted at the early-chronic stage of SCI exhibited migration away from the injury epicenter, as well as a preferential rostral localization in migration and proliferation. These data may suggest that extrinsic facto

• From an evolutionary perspective the

  2018-10-20

  

  From an evolutionary perspective, the high level of resistance of HSPCs to lentiviruses makes sense, since any major chromosomal vulnerability would have more serious implications than in progeny cells. The high-level expression of SAMHD1 in cultured HSPCs is reminiscent of the one in activated CD4+

• Molecular karyotyping identified a novel Mb gain

  2018-10-20

  

  Molecular karyotyping identified a novel 2.4Mb gain on chromosome 5p14.3 and a gain on chromosome 12p11.21, which was also found in KCL040. The gain on chromosome 5p14.3 containing a single gene, CDH18 (Cadherin-18), was also present in one of two sibling cell lines, KCL032, but not in KCL034, a th

• br Discussion We investigated whether CD

  2018-10-20

  

  Discussion We investigated whether CD133 serves as a putative epithelial stem/progenitor cell marker in adult murine prostates and explored its function in prostate regenerative capacity. We demonstrated that CD133 is not only expressed in a small fraction of prostate basal Aminoallyl-UTP Supplie

• br Conclusion br Contributions br Competing interests br Fun

  2018-10-20

  

  Conclusion Contributions Competing interests Funding This study was financially supported by German Academic Exchange Service/Federal Ministry of Education and Research (grant # D/09/04774), Center for Regenerative Therapies Dresden (Seed Grant # 09-08), Simon Fougner Hartmanns Family Fo

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